Biologics

Authors: von Plocki SC, Armbruster D, Klein K, Kämpf K, Zlinszky K, Hilbe M, Kronen P, Gruskin E, von Rechenberg B.
Journal: Vet Surg

OBJECTIVE:
To evaluate biocompatibility of biodegradable sleeves containing antimicrobial agents, designed for local drug delivery to prevent implant-related infection.
STUDY DESIGN:
Synthetic polyester sleeves (a copolymer of glycolide, caprolactone, trimethylene carbonate, lactide) were cast as thin films. The antimicrobial agents incorporated in the sleeves included gentamicin sulfate, triclosan, or a combination of these drugs.
ANIMALS:
Adult sheep (n = 15).
METHODS:

Authors: Bronzini I, Patruno M, Iacopetti I, Martinello T.
Journal: Vet J

Cell-based therapies, such as the use of mesenchymal stromal cells (MSCs), are becoming popular in veterinary medicine. When MSCs are not cryopreserved, they are shipped in suspension, but no previous studies have analyzed MSC viability during delivery. Here, the impact of several experimental shipping conditions on the number of equine blood-derived (ePB-MSC) and canine adipose-derived (cA-MSC) MSCs were evaluated. Among the different parameters tested, only time and temperature influenced MSC number during the experimental shipping conditions.

Category: Biologics
Authors: Smith TJ, Baltzer WI, Löhr C, Stieger-Vanegas SM.
Journal: VCOT

A two-year-old, 97 kg, male neutered English Mastiff was evaluated for left pelvic limb lameness of five months duration localized to the stifle joint. Following radiographic, computed tomographic and arthroscopic examination, the lameness was subsequently diagnosed as being caused by primary synovial osteochondromatosis. In total, 194 osteochondral bodies were removed using arthroscopy in combination with a mini-arthro-tomy. Histology and immunohistochemistry of the loose osteochondral fragments confirmed the diagnosis with a moderately high degree of differentiation and low cellularity.

Authors: Fitzpatrick N, Yeadon R, van Terheijden C, Smith TJ.
Journal: VCOT

Objective: To describe the clinical application of osteochondral autograft transfer procedure for the treatment of osteochondritis dissecans (OCD) of the canine medial femoral condyle and to report clinical and force plate outcomes. Methods: Osteochondral autograft transfer (OATS™ Arthrex, Naples FL, USA) instrumentation was employed in six stifle joints of five dogs. Clinical examination was performed preoperatively and at two to three weeks, six to eight weeks, 12-18 weeks and at >22 months postoperatively.

Authors: Nakladal B, Vom Hagen F, Olias P, Brunnberg L.
Journal: VCOT

This report describes a case of intraosseous lipoma in a two-year-old Leonberger. The dog was presented with a history of ten month lameness in the right forelimb. A massive swelling from the elbow to the carpus of the right forelimb was visible. Treatment with anti-inflammatory medications by the local veterinarian for ten months was unsuccessful and the dog was presented at the university clinic. Radiographic images showed that the diaphyseal part of the ulna was affected by extensive cyst-like osteolysis. Furthermore, the distal metaphysis of the radius showed cyst-like osteolytic changes.

Authors: 1. Nicholas D. Jeffery BVSc, PhD, FRCVS, Diplomate ECVN & ECVS1, 2. Nicolas Granger DVM Diplomate ECVN2
Journal: Vet Surg

No Abstract

Authors: Medl N, Guerrero TG, Hölzle L, Hässig M, Lochbrunner S, Montavon PM.
Journal: Vet Surg

OBJECTIVE: To (1) determine suction tip (intermittent and continuous mode) contamination rate in orthopedic surgery in dogs and cats; (2) examine the effect of surgical time on contamination; and (3) report bacteria isolated. STUDY DESIGN: Clinical study. SAMPLE POPULATION: Clean orthopedic surgeries (n = 50). METHODS: Surgical procedures were assigned to 1 of 2 groups: (1) continuous (n = 25) or (2) intermittent suction (n = 25). A control suction was operated in each surgery.

Authors: Webb TL, Quimby JM, Dow SW.
Journal: J Feline Med Surg

Mesenchymal stem cells (MSC) are increasingly being proposed as a therapeutic option for a variety of different diseases in human and veterinary medicine. At present, MSC are most often collected from bone marrow (BM) or adipose tissue (AT) and enriched and expanded in vitro before being transferred into recipients. However, little is known regarding the culture characteristics of feline BM-derived (BM-MSC) versus AT-derived MSC (AT-MSC). We compared BM-MSC and AT-MSC from healthy cats with respect to in vitro growth and cell surface phenotype.

Category: Biologics
Authors: Ireifej S, Marino DJ, Loughin CA, Lesser ML, Akerman M.
Journal: Vet Surg

OBJECTIVE:
To report incidence and relevance of positive intraoperative total hip replacement (THR) bacterial cultures taken at the time of surgical closure in dogs having unilateral THR.
STUDY DESIGN:
Retrospective case series.
ANIMALS:
Dogs (n = 100) with cemented (CFX), cementless (BFX), or hybrid THR.
METHODS:

Authors: Little JP, Horn TJ, Marcellin-Little DJ, Harrysson OL, West HA.
Journal: AJVR

Objective-To design and fabricate fiberglass-reinforced composite (FRC) replicas of a canine radius and compare their mechanical properties with those of radii from dog cadavers. Sample-Replicas based on 3 FRC formulations with 33%, 50%, or 60% short-length discontinuous fiberglass by weight (7 replicas/group) and 5 radii from large (> 30-kg) dog cadavers. Procedures-Bones and FRC replicas underwent nondestructive mechanical testing including 4-point bending, axial loading, and torsion and destructive testing to failure during 4-point bending.