Biologics

Authors: Akagi H, Ochi H, Kannno N, Iwata M, Ichinohe T, Harada Y, Nezu Y, Yogo T, Tagawa M, Hara Y.
Journal: VCOT

Objectives: To evaluate the efficacy of cortical allograft and fibroblast growth factor 2 (FGF-2)-impregnated autogenous cancellous bone in nonunion fracture repair in dogs. Methods: From January 2000 to August 2010, seven dogs underwent cortical allograft and FGF-2-impregnated autogenous cancellous bone implantation for treatment of a femoral nonunion following fracture. Radiographic images were used to assess healing. Results: The average length of the implanted cortical allograft was 29.1 ± 4.4 mm.

Authors: Corbin EE, Cavanaugh RP, Fick JL, McAbee KP, Powers B.
Journal: VCOT

A dog was presented with the complaint of an acute onset left pelvic limb lameness three years after a right tibial plateau levelling osteotomy had been performed. Radiographs taken at the time of presentation showed signs that were consistent with a diagnosis of an implant associated sarcoma. At revision surgery, a retained surgical sponge was identified, leading to a diagnosis of a gossypiboma. This is the first reported case of a gossypiboma as a complication of a tibial plateau levelling osteotomy surgery.

Authors: Lappalainen AK, Mölsä S, Liman A, Snellman M, Laitinen-Vapaavuori O.
Journal: JSAP

OBJECTIVE:
To determine whether the current Finnish screening method using a single flexed mediolateral view as scored by osteophyte is sufficient to diagnose mild elbow dysplasia in Labrador retrievers and to determine if an additional craniocaudal oblique projection would result in improvement in the screening protocol.
MATERIALS AND METHODS:
Thirteen dogs with one mildly affected elbow joint and one elbow joint without radiological evidence of osteophytes were studied. Radiographic and computed tomography studies were performed and the results compared with each other.
RESULTS:

Category: Biologics - Hip - Imaging
Authors: Zhang N, Dietrich MA, Lopez MJ.
Journal: Vet Surg

OBJECTIVE:
To identify the optimum intra-articular multipotent stromal cell (MSC) tissue source in the canine stifle.
STUDY DESIGN:
Experimental.
SAMPLE POPULATION:
Infrapatellar adipose tissue, synovium lining the joint capsule, and synovium surrounding the cranial cruciate ligament (CrCL) from normal stifles of 6 dogs.
METHODS:

Authors: Herten M, Grassmann JP, Sager M, Benga L, Fischer JC, Jäger M, Betsch M, Wild M, Hakimi M, Jungbluth P.
Journal: VCOT

Autologous bone marrow plays an increasing role in the treatment of bone, cartilage and tendon healing disorders. Cell-based therapies display promising results in the support of local regeneration, especially therapies using intra-operative one-step treatments with autologous progenitor cells. In the present study, bone marrow-derived cells were concentrated in a point-of-care device and investigated for their mesenchymal stem cell (MSC) characteristics and their osteogenic potential.Bone marrow was harvested from the iliac crest of 16 minipigs.

Category: Biologics
Authors: Etter SW, Ragetly GR, Bennett RA, Schaeffer DJ.
Journal: JAVMA

Objective-To compare surgical site infection and inflammation rates between the use of nonimpregnated (polydioxanone and poliglecaprone 25) versus triclosan-impregnated (polydioxanone and poliglecaprone 25) suture for incisional closure in dogs undergoing a standardized orthopedic procedure (tibial plateau leveling osteotomy [TPLO]). Design-Retrospective cohort study. Animals-283 dogs that underwent TPLO between November 2005 and December 2009.

Authors: Pinel CB, Pluhar GE.
Journal: Can Vet J

Databases (2001-2008) for cases in which recombinant bone morphogenetic protein (rhBMP) was used to aid in management of orthopedic disease were reviewed and cases were categorized as non-unions, delayed unions, and cases expected to heal with difficulty. If follow-up in the medical record was < 6 mo for live animals, owners were surveyed by telephone. Thirteen cases (11 dogs, 2 cats) were identified; OP-1 (rhBMP-7) was used in 3 cases and INFUSE (rhBMP-2) in 10.

Authors: Lo AJ, Goldschmidt MH, Aronson LR.
Journal: J Fel Med Surg

A 4-year-old castrated male Russian Blue cat was evaluated for acute right hind limb lameness 18 months after receiving a renal transplant. Radiographs showed a subluxated right femoral head and lysis of the acetabulum and femoral neck. A femoral head and neck ostectomy was performed on the right coxofemoral joint. Histologic evaluation of the right femoral head revealed lesions indicative of a chronic, granulomatous osteomyelitis and periostitis associated with an intralesional Mycobacterium species.

Category: Arthritis - Biologics
Authors: Hodgkiss-Geere HM, Argyle DJ, Corcoran BM, Whitelaw B, Milne E, Bennett D, Argyle SA.
Journal: Vet J

Mesenchymal stem cells (MSCs) have potential for use in regenerative therapeutics, since they are capable of multi-lineage differentiation. In this study, primary canine MSCs (cMSCs) were isolated from bone marrow aspirates and characterised using marker expression and morphology. cMSCs expressed CD44 and STRO-1, but not CD34 or CD45. Morphologically, cMSCs were similar to previously described MSCs and were capable of chondrocyte differentiation towards articular type cartilage, characterised by increased collagen type II vs. collagen type I expression and expression of Sox-9.

Category: Biologics